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RPMI-1640 Medium

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產(chǎn)品型號:R5886-100ML

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廠商性質(zhì):代理商

所  在  地:北京市

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更新時間:2023-07-11 14:16:40瀏覽次數(shù):739次

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供貨周期 現(xiàn)貨 規(guī)格 100ML
貨號 R5886-100ML 應用領域 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè),綜合
RPMI-1640 Medium
HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture.

RPMI-1640 Medium

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested;pH>7.2.

components:L-glutamine: no;NaHCO3: yes;sodium pyruvate: no;HEPES: 25 mM;phenol red: yes.

General description:RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

Application:

RPMI-1640 Medium has been used:

to culture human lymphoblastoid cell line cells

prostate cancer cells

monocytes from human blood



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